Fig. 2

Lysosomal Storage in snapc1b Mutants Compromises Physiological Efferocytosis

(A) Still images from confocal video of green fluorescent macrophages in snapc1b mutant larval and WT sibling brains. Time of image is indicated in minutes. Arrows mark pseudopodia; arrowheads mark vacuoles. Vertical dotted red line indicates the time point immediately following phagocytic event. Scale bar, 15 µm.

(B) Speed of WT and snapc1b mutant macrophages from the confocal video in (A). Average speed before and after the phagocytic events are indicated by a horizontal blue line. Green dots correspond to time points in the images shown in (A).

(C) Migration of normal and vacuolated macrophages from the same animal to CCL2 injected into the HBV.

(D) Representative confocal image of red fluorescent macrophages stained with acridine orange (AO) in brains of snapc1b mutant larvae and WT siblings at 3 dpf. Arrow marks a wild-type macrophage with very little AO staining. Arrowhead marks a rare AO positive macrophages seen in WT brains. Scale bar, 30 µm.

(E and F) Confocal images (E) and quantification (F) of green fluorescent acridine-orange-stained unengulfed cell debris in the brains of snapc1b mutant larvae and WT siblings at 5 dpf. Scale bar, 150 µm. Images in (E) denoted as red data points in (F).

Statistical significance was assessed by Student’s t test (B and F) and paired t test (C). See also Figure S3.