FIGURE

Fig. 1

ID

ZDB-FIG-160412-12

Publication

Wan et al., 2016 - Transcriptome analysis reveals a ribosome constituents disorder involved in the RPL5 downregulated zebrafish model of Diamond-Blackfan anemia

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Fig. 1

Hemoglobin staining of embryos injected with RPL5 MO and the effectiveness of RPL5 MO. a-b Embryos co-injected with 25 ng RPL5:egfp DNA and 0.25 ng control MO produced green fluorescent protein (a), and the expression of the green fluorescent fusion protein was inhibited by co-injection with 0.25 ng RPL5 MO (b). c-e The different phenotype and its ratio of embryos injected with 0.25 ng RPL5 MO f-k The O-staining results revealed a drastic reduction in the number of hemoglobin-stained blood cells when RPL5 was knocked down (f and i show the control; g and j show the RPL5 knockdown), and this was partially rescued by coinjection with P53 MO (h and k). a, b, c, d, e,i, j and k show the lateral view; f, g, and h show the ventral view

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-rpl5a AB + MO1-rpl5a + MO4-tp53
Knockdown Reagents:	MO1-rpl5a MO4-tp53
Observed In:	embryo development head heart nucleate erythrocyte nucleate erythrocyte post-vent region
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Med. Genomics