FIGURE

Fig. 5

ID

ZDB-FIG-160308-16

Publication

Ponomareva et al., 2016 - Charcot-Marie-Tooth 2b associated Rab7 mutations cause axon growth and guidance defects during vertebrate sensory neuron development

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Fig. 5

Central axon guidance defects in transgenics expressing CMT2b Rab7 mutants. a Lateral (top two panels) and 3D rotation dorsal (bottom two panels) views of TagRFP-caax labeled RB neuron in Tg(-3.1ngn:gfp-Rab7) embryo at 23 hpf showing ascending and descending ipsilateral projections of central axons. Two bilateral rows of RB cells (green) are best seen in dorsal views. b-c Lateral (top two panels) and 3D rotation dorsal (bottom two panels) views of TagRFP-caax labeled RB neurons in Tg(-3.1ngn:gfp-Rab7 L129F) embryos at 23 hpf. b Misguided descending central axon (yellow arrow) turns around to travel anteriorly and dorsally from its normal pathway. c Misguided central axon (yellow arrowhead) crosses dorsal midline to join the contralateral central fascicle. All views anterior to the left. Scale bar = 40 µm. d Quantification of number of neurons with central axon guidance errors. Cont Rab7 = Tg(-3.1ngn:gfp-Rab7): 10 neurons in 7 embryos; Tg(-3.1ngn:gfp-Rab7 L129F): 43 cells in 43 embryos, *p = 0.05; Tg(-3.1ngn:gfp-Rab7K157M): 48 cells in 27 embryos, p = 0.2; Chi Square test

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	uw12Tg uw13Tg
Observed In:	sensory neuron axon guidance
Stage:	26+ somites

Phenotype Detail

Acknowledgments

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