FIGURE
Fig. 5
- ID
- ZDB-FIG-160229-8
- Publication
- Jungke et al., 2016 - Generation of a conditional lima1a allele in zebrafish using the FLEx switch technology
- Other Figures
- All Figure Page
- Back to All Figure Page
Fig. 5
Cre-mediated re-inversion of SAGFLEx. (A) Schematic representation of the events of SAGFLEx integrated into intron 2 of the lima1a locus in Tg(FLEx-lima1a:GFP) after Cre expression. The positions of the primers used for the allele-specific PCR (E2-for/pA-for/GFP-rev) are indicated. (B) Expression of Cre in rhombomeres 3 and 5 (r3/5) of the developing hindbrain results in the restoration of native GFP fluorescence. (C) Allele-specific PCR of GFP-positive and negative Tg(FLEx-lima1:GFP)inv embryos with the primers indicated above. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Genesis