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Fig. 5
Cre-mediated re-inversion of SAGFLEx. (A) Schematic representation of the events of SAGFLEx integrated into intron 2 of the lima1a locus in Tg(FLEx-lima1a:GFP) after Cre expression. The positions of the primers used for the allele-specific PCR (E2-for/pA-for/GFP-rev) are indicated. (B) Expression of Cre in rhombomeres 3 and 5 (r3/5) of the developing hindbrain results in the restoration of native GFP fluorescence. (C) Allele-specific PCR of GFP-positive and negative Tg(FLEx-lima1:GFP)inv embryos with the primers indicated above.
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