Fig. 3

The Wound Border Zone Can Be Subdivided Based on Distinct Expression Profiles

(A) Cluster analysis on all genes with expression peaks in greater than three consecutive sections in the border zone (sections 8–18) of the 3 dpi heart identifies two sub-regions showing different expression patterns. Color spectrum indicates Z score. BZinj and BZmyo are marked by dashed yellow lines.

(B) Expression traces from tomo-seq data on cryoinjured heart (3 dpi) for selected genes showing peak expression in the BZinj region.

(C) Expression traces from tomo-seq data on cryoinjured heart (3 dpi) for selected genes showing peak expression in the BZmyo region.

(D) Expression traces from tomo-seq data and in situ hybridization on cryoinjured heart (3 dpi) for the myosin heavy chain (MHC) genes vmhc and vmhc-like (vmhcl). Scale bar, 100 µm.

(E) Ventricular myosin heavy chain (Vmhc) expression is detected in the developing heart of myl7:GFP transgenic embryos at 2 days post fertilization (arrow), but not in uninjured adult hearts. Vmhc is again expressed in GFP^low cardiomyocytes at 7 dpi. In LUT (lookup table) images, GFP intensity below 30% of signal intensity is displayed in red (GFP^low) while the rest is displayed in green (GFP^high). Note the overlapping expression pattern between GFP^low area (depicted as GFP^low selection) and Vmhc. Average Vmhc signal intensity in uninjured, GFP^low, and GFP^high cardiomyocytes at 7 dpi is plotted on the right. Error bars represent SEM. Student’s t test, ***p = 0.0008 (uninjured versus GFP^low), **p = 0.002 (uninjured versus GFP^high), **p = 0.004 (GFP^low versus GFP^high). Scale bar, 50 µm.