FIGURE

Fig. 8

ID

ZDB-FIG-160215-23

Publication

Yokota et al., 2015 - Endothelial Ca(2+) oscillations reflect VEGFR signaling-regulated angiogenic capacity in vivo

Other Figures

All Figure Page

Back to All Figure Page

Fig. 8

Dll4 attenuates Ca²⁺ oscillations in the entire DA before ISV sprouting.

(A) 3D-rendered time-sequential images of Tg(fli1:Gal4FF);(UAS:GCaMP7a);(fli1:H2B-mC) embryos before ISV sprouting injected with dll4 MO (17 ss). Yellow dashed lines indicate positions of somite boundaries. (B) The DA is subdivided into three regions (Region 1–3) as illustrated in the scheme (left). The fluorescence changes in GCaMP7a (ΔF/F₀) of individual ECs from A are shown as separated graphs (Region 1–3) as in Figure 3B. A representative graph of two ECs at each region is shown. (C) Quantification of Ca²⁺ oscillation frequency (upper) and mean ΔF/F₀ (lower) in ECs of the indicated regions within the DA in control MO- or dll4 MO-injected embryos before vessel sprouting (17–19 ss). Horizontal lines represent mean ± s.d. (n e 16). Scale bar, 10 mm in A. *p < 0.05, **p < 0.01, ***p < 0.001. DA, dorsal aorta.

Expression Data

Genes:	GCaMP mCherry
Fish:	ncv31Tg; ubs4Tg; zf415Tg + MO3-dll4
Knockdown Reagent:	MO3-dll4
Anatomical Terms:	endothelial cell nucleus endothelial tip cell
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ncv31Tg; ubs4Tg; zf415Tg + MO3-dll4
Knockdown Reagent:	MO3-dll4
Observed In:	endothelial tip cell
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife