FIGURE

Fig. 5

ID

ZDB-FIG-160215-21

Publication

Yokota et al., 2015 - Endothelial Ca(2+) oscillations reflect VEGFR signaling-regulated angiogenic capacity in vivo

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Fig. 5

Ca²⁺ oscillations in stalk cells during budding from the DA.

(A) 3D-rendered time-sequential images of Tg(fli1:Gal4FF);(UAS:GCaMP7a);(fli1:H2B-mC) embryos during stalk cell budding from the DA (29 ss). Green and orange arrowheads indicate tip and stalk cells, respectively. (B) The fluorescence changes in GCaMP7a (ΔF/F₀) of individual ECs from A indicated by arrowheads (green, orange, light gray, dark gray, and black) at the left panel are shown as a graph. (C) Quantification of Ca²⁺ oscillatory activity in untreated and ki8751-treated embryos during stalk cell budding from the DA as in A and Figure 5—figure supplement 1A, respectively. Graphs show Ca²⁺ oscillation frequency (left) and mean ΔF/F₀ (right) in tip cells, stalk cells and other ECs within the DA in untreated and ki8751-treated embryos (Untreated, n e 10; ki8751-treated, n e 13). (D) Stalk cells that are budding from the DA have significant Vegfr2 activity, albeit weaker than that in tip cells. Scale bar, 10 µm in A. *p < 0.05, **p < 0.01, ***p < 0.001; NS, not significant. DA, dorsal aorta.

Expression Data

Genes:	GCaMP mCherry
Fish:	ncv31Tg; ubs4Tg; zf415Tg
Anatomical Terms:	blood vessel endothelial cell cytoplasm endothelial cell nucleus endothelial tip cell cytoplasm
Stage Range:	26+ somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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