FIGURE

Fig. 2

ID

ZDB-FIG-160203-10

Publication

Samsa et al., 2015 - Cardiac contraction activates endocardial Notch signaling to modulate chamber maturation in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Cardiac contraction is required for endocardial Notch activation and notch1b transcription. (A) Schematic diagram of morpholino (MO) gene knockdown experiment, in which double transgenic Tg(Tp1:EGFP); Tg(myl7:dsRed) embryos were injected with (B,C) control, (D,E) tnnt2a or (F,G) notch1b morpholinos and imaged at 48-50hpf. (B,D,F) Representative whole-mount images of Notch reporter with cardiac regions highlighted by circles. (C,E,G) Confocal maximal intensity projections of the hearts shown in (B,D,F) with cardiomyocytes labeled in red, (C′,E′,G′) Notch reporters in green and (C′′,E′′,G′′) colocalized signal in yellow. Minimal colocalization indicates that Notch activation is in endocardial cells. (H,I) Whole-mount notch1b riboprobe hybridization in (H) control and (I) tnnt2a^-/- embryos, with the heart outlined in red. Scale bar: 50µm. a, atrium; v, ventricle.

Expression Data

Genes:	DsRed2 EGFP notch1b
Fish:	WT tnnt2a^b109/b109 um14Tg; vc6Tg um14Tg; vc6Tg + MO1-tnnt2a um14Tg; vc6Tg + MO2-notch1b
Knockdown Reagents:	MO1-tnnt2a MO2-notch1b
Anatomical Terms:	atrioventricular canal atrium brain cardiac muscle cell heart ventricular endocardium
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tnnt2a^b109/b109 um14Tg; vc6Tg + MO1-tnnt2a um14Tg; vc6Tg + MO2-notch1b
Knockdown Reagents:	MO1-tnnt2a MO2-notch1b
Observed In:	heart heart contraction Notch signaling involved in heart development
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development