FIGURE

Fig. S2

ID

ZDB-FIG-160108-2

Publication

Tuttle et al., 2014 - Rabconnectin-3a regulates vesicle endocytosis and canonical Wnt signaling in zebrafish neural crest migration

Other Figures

All Figure Page

Back to All Figure Page

Fig. S2

Rescue of rbc3a-MO1-injected embryos with full-length rbc3a mRNA. (A-C) Fluorescent images of live sox10:gfp transgenics, dorsal views, anterior to the left: (A) wild-type (WT), (B) rbc3a-MO1 injected, and (C) co-injected with full length rbc3a mRNA. (D) Number of GFP+ cells aggregated at the dorsal midilne at 24 hpf and located between the otic vesicle and MHB along the A-P axis. Injection of 100 pg rbc3a mRNA significantly rescued the number of GFP+ cells from 27.4±2.7 in rbc3a-MO1-injected embryos (n = 8) to 10.8±3.0 cells in mRNA+MO injected embryos (p<0.001, n = 8). Injection of 100 pg mRNA alone (n = 8) had no effect on the number of midline GFP+ cells compared to 2.3±1.7 in wild-type embryos (n = 9). Error bars represent ± SEM. Scale bar, 100 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ir937Tg + MO1-dmxl2 + MO1-tp53
Knockdown Reagents:	MO1-dmxl2 MO1-tp53
Observed In:	neural crest cell neural crest cell migration
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.