FIGURE

Fig. S6

ID

ZDB-FIG-151211-4

Publication

Jia et al., 2015 - Mutation of kri1l causes definitive hematopoiesis failure via PERK-dependent excessive autophagy induction

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Fig. S6

Mutants^cas002 phenotype in Tg (cmyb:egfp) background. (A-F) Representative confocal images of EGFP⁺ cells in WT and mutant^cas002 within Tg (cmyb: egfp) transgenic background. Scale bars: 50 μm. (G) Quantitative analysis of A-F. The number ofEGFP⁺ cells is comparable in mutant embryos at 2 dpf (p=0.7131). The numbers of EGFP⁺ cells are decreased in mutant embryos at 3 dpf (p<0.0001) and 4 dpf (p<0.0001). (H) Sequencing electropherogram of the mutation site of kri1l gene genomic DNA from 5 wild-type strains. Genomic DNA were extracted each from 20-30 embryos of TU, AB, WIK, Longfin and Shanghai strains, and were then sequenced for kri1l gene. This point mutation (T-G) in kri1l^cas002 was not found in all five wild-type strains, indicating that T-G transition at the site is not a SNP.

Expression Data

Gene:	EGFP
Fish:	zf169Tg kri1^{cas002/cas002}; zf169Tg
Anatomical Term:	hematopoietic stem cell
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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