FIGURE

Fig. 1

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ZDB-FIG-151203-1

Publication

Kagermeier-Schenk et al., 2011 - Waif1/5T4 Inhibits Wnt/β-Catenin Signaling and Activates Noncanonical Wnt Pathways by Modifying LRP6 Subcellular Localization

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Fig. 1

Zebrafish waif1a Is a Direct Universal β-Catenin Target Gene Encoding a Transmembrane Protein Localized to the Plasma Membrane

(A) Domain structure of zebrafish Waif1a and human Waif1/5T4.

(B) Localization of GFP-tagged zebrafish Waif1a in enveloping cells (EVL) of sphere stage zebrafish embryos and in HEK293T cells.

(C) waif1a expression assessed by in situ hybridization in zebrafish embryos expressing Wnt8, Dkk1, or Axin1 (n [90%] = Wnt8, 13 of 13 embryos, Dkk1 18 of 18, Axin1 15 of 15; n [3 somites] = Dkk1 15 of 15, Axin1 18 of 20; n [24 hpf] = Dkk1 15 of 15, Axin1 14 of 14).

(D) waif1a upregulation in embryos injected with GR Lef ΔNβ-cat RNA and treated with dexamethasone (Dex, 24 of 24 embryos) and in embryos pretreated with Cycloheximide (Chx, 22 of 22 embryos).

(E) GFP expression in GFP RNA-injected embryos after 1 hr of EtOH or Chx treatment.

See also Figure S1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 21(6), Kagermeier-Schenk, B., Wehner, D., Ozhan-Kizil, G., Yamamoto, H., Li, J., Kirchner, K., Hoffmann, C., Stern, P., Kikuchi, A., Schambony, A., and Weidinger, G., Waif1/5T4 Inhibits Wnt/β-Catenin Signaling and Activates Noncanonical Wnt Pathways by Modifying LRP6 Subcellular Localization, 1129-43, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell