Fig. S8
- ID
- ZDB-FIG-120201-34
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- Kagermeier-Schenk et al., 2011 - Waif1/5T4 Inhibits Wnt/β-Catenin Signaling and Activates Noncanonical Wnt Pathways by Modifying LRP6 Subcellular Localization
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Waif1a enhances noncanonical effects of Dkk1, related to figure 8. (A) xwaif1 MO is functional. Injection of xwaif1a.2 MO (1.6 pmol) downregulates expression of a myc-tagged xwaif1a.2 construct harboring the endogenous xwaif1a.2 5′ UTR, while it has no effect on a xwaif1a.2-myc construct lacking the xwaif1a.2 5′UTR. (B) xdkk1 MO is functional. anti-XDkk1 antibody was used to detect levels of endogenous Xenopus Dkk1 protein in lysates of embryos injected with 1.6 pmol xdkk1 MO or control MO. (C) waif1a rescues CE defects induced by dkk1 knockdown (3 ng dkk1 MO) in zebrafish embryos. CE defects are evident by compressed myoD-positive somites (arrowheads) and widened krox20 and pax2.1 positive neural expression domains (brackets) at the 15 somite stage. Coinjection of 60 pg waif1a RNA completely rescues this phenotype (22/27 embryos). (D) Waif1a rescues body length defects caused by hypomorphic knockdown of zebrafish dkk1. Injection of 3 ng of dkk1 MO results in reduced body length (measured from the otic vesicle to the tip of the tail) at 24 hpf, which can be completely rescued by coinjection of 60 pg waif1a RNA. |
Reprinted from Developmental Cell, 21(6), Kagermeier-Schenk, B., Wehner, D., Ozhan-Kizil, G., Yamamoto, H., Li, J., Kirchner, K., Hoffmann, C., Stern, P., Kikuchi, A., Schambony, A., and Weidinger, G., Waif1/5T4 Inhibits Wnt/β-Catenin Signaling and Activates Noncanonical Wnt Pathways by Modifying LRP6 Subcellular Localization, 1129-43, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell