FIGURE

Fig. 5

ID

ZDB-FIG-151130-7

Publication

Kiesow et al., 2015 - Junb controls lymphatic vascular development in zebrafish via miR-182

Other Figures

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Fig. 5

Ectopic miR-182 restores PL formation in Junb morphants.

(a) Left panel, bright field images showing overall morphology of junba and junbb (2 + 2 ng) double morphants injected with or without Neg-Mimic (40 pg, n = 127) or 182-Mimic (40 pg, n = 109). Middle panel: Confocal images of indicated trunk region in Tg(fli1:EGFP)^y1 embryos at 72 hpf. Right panel, enlarged view of trunk region marked by the yellow rectangle in the middle panel. DA, dorsal aorta, PCV, posterior cardinal vein and ISV, intersegmental vessels. PL is marked with yellow arrows or if absent with asterisks. Panels are lateral view; dorsal is up, anterior to the left. Scale bars: 500 µm (black); 50 µm (white). (b) Percentage of embryos injected with MOs and Mimics as indicated and (c) for a second set of junb-MOs: junba-MO2 + junbb-MO2 (2 ng + 2 ng, n = 112), with or without Neg-Mimic (40 pg, n = 145) or 182-Mimic (40 pg, n = 119). ***P < 0.001, Mann-Whitney U-test.

Expression Data

Gene:	EGFP
Fish:	y1Tg y1Tg + MO2-junba + MO3-junbb + MO4-tp53
Knockdown Reagents:	MO2-junba MO3-junbb MO4-tp53
Anatomical Terms:	dorsal aorta dorsal longitudinal anastomotic vessel intersegmental vessel posterior cardinal vein vascular lymphangioblast
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg + MO2-junba + MO3-junbb + MO4-tp53 y1Tg + MO3-junba + MO4-junbb + MO4-tp53
Knockdown Reagents:	MO2-junba MO3-junba MO3-junbb MO4-junbb MO4-tp53
Observed In:	vascular lymphangioblast
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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