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Fig. 5 Ectopic miR-182 restores PL formation in Junb morphants.
(a) Left panel, bright field images showing overall morphology of junba and junbb (2 + 2 ng) double morphants injected with or without Neg-Mimic (40 pg, n = 127) or 182-Mimic (40 pg, n = 109). Middle panel: Confocal images of indicated trunk region in Tg(fli1:EGFP)y1 embryos at 72 hpf. Right panel, enlarged view of trunk region marked by the yellow rectangle in the middle panel. DA, dorsal aorta, PCV, posterior cardinal vein and ISV, intersegmental vessels. PL is marked with yellow arrows or if absent with asterisks. Panels are lateral view; dorsal is up, anterior to the left. Scale bars: 500 µm (black); 50 µm (white). (b) Percentage of embryos injected with MOs and Mimics as indicated and (c) for a second set of junb-MOs: junba-MO2 + junbb-MO2 (2 ng + 2 ng, n = 112), with or without Neg-Mimic (40 pg, n = 145) or 182-Mimic (40 pg, n = 119). ***P < 0.001, Mann-Whitney U-test.