FIGURE

Fig. 2

ID

ZDB-FIG-151030-13

Publication

Miesfeld et al., 2015 - Yap and Taz regulate retinal pigment epithelial cell fate

Other Figures

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Fig. 2

yap^nl13/nl13 mutants exhibit coloboma. (A) Splice site variants elicited by the yap^nl13 allele. The mutation results in aberrant splicing and an early stop codon in the transactivation domain for all described products. (B) Protein schematics for wild-type and predicted mutant yap^nl13 allele variants. (C) Western blot showing the loss of full-length Yap in yapⁿ^l^13/nl13 mutants. A smaller protein product is detectable at higher levels in the mutant (~40 kDa). (D-D′′,G-G′′) 3dpf wild-type and yap^nl13/nl13 embryos showing coloboma (arrows) in the absence of other overt phenotypes. Plastic sections of wild-type (D′′) and mutant (G′′) eyes show the coloboma (arrow) phenotype and RPE deficits that are sometimes observed in the ventral retina. (E,F,H,I) Sections showing Yap and Taz proteins (green) in wild-type and yapⁿ^l^13/nl13 mutant eyes. Red counterstain (TO-PRO3) shows nuclei.

Expression Data

Antibodies:	Ab1-wwtr1 Ab1-yap1
Fish:	WT yap1^nl13/nl13
Anatomical Terms:	eye whole organism
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	yap1^nl13/nl13
Observed In:	eye retinal pigmented epithelium
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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