Fig. 6

TARS is involved in vascular development in the zebrafish.

(a) Representative confocal images (20× magnification) of control and TARS morphant zebrafish ISVs at 24 and 48 hpe. Embryos were injected with a TARS morpholino (1.5 µM) at the one to four cell stage. Fish were manually dechorionated at 24 h after fertilization and imaged at 24 and 48 hpe. Arrows and asterisks denote the location of ectopic branches and missing/incomplete vessels respectively. (b,c) Quantification of ectopic branching (b) and missing/incomplete ISVs (c) from control and morphant zebrafish within a region of encompassing five ISVs anterior and posterior to the yolk extension; mean ± SEM, n ≥ 29, ^#*p < 0.0001 relative to uninjected controls at 24 and 48 hpe respectively (one-way ANOVA, Tukey Test). See Supplementary Figure S10 for morpholino validation and morphological effects.