PUBLICATION
Aminoacyl-tRNA synthetase dependent angiogenesis revealed by a bioengineered macrolide inhibitor
- Authors
- Mirando, A.C., Fang, P., Williams, T.F., Baldor, L.C., Howe, A.K., Ebert, A.M., Wilkinson, B., Lounsbury, K.M., Guo, M., Francklyn, C.S.
- ID
- ZDB-PUB-150815-4
- Date
- 2015
- Source
- Scientific Reports 5: 13160 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Amino Acyl-tRNA Synthetases/metabolism*
- Angiogenesis Inhibitors/administration & dosage*
- Angiogenesis Inhibitors/chemistry
- Angiogenic Proteins/metabolism*
- Animals
- Dose-Response Relationship, Drug
- Enzyme Activation
- Macrolides/antagonists & inhibitors*
- Neovascularization, Physiologic/drug effects*
- Neovascularization, Physiologic/physiology*
- Zebrafish
- PubMed
- 26271225 Full text @ Sci. Rep.
- CTD
- 26271225
Citation
Mirando, A.C., Fang, P., Williams, T.F., Baldor, L.C., Howe, A.K., Ebert, A.M., Wilkinson, B., Lounsbury, K.M., Guo, M., Francklyn, C.S. (2015) Aminoacyl-tRNA synthetase dependent angiogenesis revealed by a bioengineered macrolide inhibitor. Scientific Reports. 5:13160.
Abstract
Aminoacyl-tRNA synthetases (AARSs) catalyze an early step in protein synthesis, but also regulate diverse physiological processes in animal cells. These include angiogenesis, and human threonyl-tRNA synthetase (TARS) represents a potent pro-angiogenic AARS. Angiogenesis stimulation can be blocked by the macrolide antibiotic borrelidin (BN), which exhibits a broad spectrum toxicity that has discouraged deeper investigation. Recently, a less toxic variant (BC194) was identified that potently inhibits angiogenesis. Employing biochemical, cell biological, and biophysical approaches, we demonstrate that the toxicity of BN and its derivatives is linked to its competition with the threonine substrate at the molecular level, which stimulates amino acid starvation and apoptosis. By separating toxicity from the inhibition of angiogenesis, a direct role for TARS in vascular development in the zebrafish could be demonstrated. Bioengineered natural products are thus useful tools in unmasking the cryptic functions of conventional enzymes in the regulation of complex processes in higher metazoans.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping