Fig. 5

Development of Purkinje cells can be rescued in klf8 morphants by co-injection of p53 MO^sp or klf8 mRNA. (A–D) Expression of roraa at 72 hpf is observed in WT embryos (A) and embryos injected with klf8-4mmMO1 (B), but not in embryos injected with klf8-MO1^atg (C, arrowhead); however, expression can be rescued by co-injection of p53 MO^sp (D). (E–H) Expression of Pvalb7 at 72 hpf is observed in WT embryos (E) or embryos injected with klf8-4mmMO1 (F), but not in embryos injected with klf8-MO1^atg (G); however, expression can be rescued by co-injection of p53 MO^sp (H). (I–L) Expression of Pvalb7 at 72 hpf is observed in WT embryos (I), but is lost in embryos injected with klf8-MO1^atg (J); expression can be rescued by co-injection with different amounts of klf8 mRNA (L), but not with LacZ mRNA (K). (M) Percentage of embryos of the indicated backgrounds lacking roraa expression. (N–O) Percentage of embryos of the indicated backgrounds lacking Pvalb7 expression. Dorsal views of embryos are shown. Significance was determined using Student′s t-test. ***p < 0.001. Error bars indicate standard errors. Pc, Purkinje cells.