FIGURE

Fig. S11

ID

ZDB-FIG-150915-69

Publication

Gao et al., 2015 - TopBP1 Governs Hematopoietic Stem/Progenitor Cells Survival in Zebrafish Definitive Hematopoiesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. S11

lmo2 promoter-induced ectopic expression of wild-type topbp1 can partially rescue HSPCs defect in topbp1^cas003 mutant.

(A) Fluorescence observation of embryos injected with lmo2: topbp1^WT transient transgenesis construct. P2A-mCherry fragment was added after topbp1^WT as an indicator. The mCherry positive cells could be detected in the CHT region at 5dpf in the injected embryos instead of non-injected embryos. Arrow heads represent the melanocytes; arrows indicate the mCherry+ cells. (B-E′) The c-myb WISH analysis of topbp1^cas003 mutant embryos with ectopic expression of ubi: topbp1WT or lmo2: topbp1^WT. Ectopic expression of lmo2: topbp1^WTcould partially rescue the c-myb expression in topbp1^cas003 mutants at 5dpf. (B) Quantitation of the rescue assay. (C′-E′) Enlarged views of the CHT regions in the left column.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.