Fig. S11

Fig. S11 lmo2 promoter-induced ectopic expression of wild-type topbp1 can partially rescue HSPCs defect in topbp1^cas003 mutant.

(A) Fluorescence observation of embryos injected with lmo2: topbp1^WT transient transgenesis construct. P2A-mCherry fragment was added after topbp1^WT as an indicator. The mCherry positive cells could be detected in the CHT region at 5dpf in the injected embryos instead of non-injected embryos. Arrow heads represent the melanocytes; arrows indicate the mCherry+ cells. (B-E′) The c-myb WISH analysis of topbp1^cas003 mutant embryos with ectopic expression of ubi: topbp1WT or lmo2: topbp1^WT. Ectopic expression of lmo2: topbp1^WTcould partially rescue the c-myb expression in topbp1^cas003 mutants at 5dpf. (B) Quantitation of the rescue assay. (C′-E′) Enlarged views of the CHT regions in the left column.