FIGURE

Fig. S7

ID

ZDB-FIG-150708-13

Publication

Sato et al., 2015 - Neuregulin 1 Type II-ErbB Signaling Promotes Cell Divisions Generating Neurons from Neural Progenitor Cells in the Developing Zebrafish Brain

Other Figures

All Figure Page

Back to All Figure Page

Fig. S7

MO^nrg1IIatg specifically suppresses ectopic expression of NRG1-II.

A. A schematic structure of an expression plasmid CMV:nrg1II-EGFP (top), a part of the nucleotide and amino acid sequences encoding 5′ untranslated and coding regions in the first exon of nrg1-II (middle), and the target sequence of MO^nrg1IIatg (bottom, red arrow). B. Representative 74-hpf embryos co-injected with CMV:nrg1II-EGFP expression plasmid and MO^nrg1IIatg or the control MO^nrg1IIatg5m shown in a lateral view. Green fluorescence in yolk of MO^nrg1IIatg-injected embryos is autoflurescence (arrowhead). Scale bar, 500 µm. C. Quantification of ratios of GFP-positive embryos co-injected with CMV:nrg1II-EGFP and MO^nrg1IIatg or the control MO^nrg1IIatg5m at 25 hpf. No GFP-positive embryos were detected for MO^nrg1IIatg-injected embryos under a fluorescent dissection microscopy. (mean ± s.e.m.; nrg1IIatg5m, 0.56 ± 0.08, n = 183 embryos by 4 injections, nrg1IIatg, 0.00 ± 0.00, n = 98 embryos by 4 injections).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One