Fig. 2

Reduced Sleep in aanat2^−/− Zebrafish Does Not Require an Entrained Circadian Rhythm

(A and B) Larvae raised and tested in DD do not exhibit circadian locomotor activity or sleep rhythms. aanat2^−/− larvae are more active (A, E, and F) and sleep less (B–D) than sibling controls. (G–L) aanat2^−/− larvae treated with DMSO vehicle control are more active (G, K, and L) and sleep less (H–J) than aanat2^+/− siblings. aanat2^−/− larvae treated with 10 μM melatonin exhibit locomotor activity and sleep indistinguishable from their melatonin-treated aanat2^+/− siblings (G–L). Data are from one representative experiment (A, B, G, and H), or combined from two (I–L) or three (C–F) experiments. Bar graphs represent mean ± SEM for the fifth and sixth days and nights of development. n, number of larvae. p < 0.01 compared to aanat2^−/− (C–F) or aanat2^−/− DMSO (I–L) by Dunnett’s test. See also Figure S3.