Fig. 1

aanat2^−/− Larvae Sleep Less at Night

(A–F) aanat2^−/− larvae sleep less (B and D) and are more active (A and F) than sibling controls at night, but not during the day (A, B, C, and E). (G–L) Arrow indicates addition of 10 μM melatonin on day 6. aanat2^−/− sleep and locomotor activity phenotypes of night 5 are absent on night 6 (G, H, J, and L). During the day, exogenous melatonin decreases locomotor activity (G and K) and increases sleep (H and I) for both genotypes. Day 6 Pre and Post refer to periods before and after melatonin addition. Total sleep amount can vary for nights 5 and 6 of development, so comparisons between different genotypes should be made on the same night. Data are from one representative experiment (A, B, G, and H) or combined from two (I–L) or three (C–F) experiments. Bar graphs represent mean ± SEM. n, number of larvae. ^∗∗p < 0.01 compared to aanat2^−/− (C–F), each genotype Day 6 Post compared to Day 6 Pre (I and K), or the indicated comparisons (J and L) by Dunnett’s (C–F) or Tukey’s (I–L) test. See also Figure S1.