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Fig. 3

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ZDB-FIG-150612-3

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Hall et al., 2013 - Immunoresponsive Gene 1 Augments Bactericidal Activity of Macrophage-Lineage Cells by Regulating β-Oxidation-Dependent Mitochondrial ROS Production

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Fig. 3

Glucocorticoid Receptor-Mediated Signaling Helps Regulate Infection-Responsive irg1 Expression within Macrophage-Lineage Cells through Induction of cebpβ Expression

(A and B) Expression of irg1 within infected DMSO (control)- and RU-486-treated (250 µM) larvae, respectively, at 1 hpi.

(C) Quantification of irg1⁺ cells within individual infected larvae treated with DMSO (control), 125 µM RU-486, or 250 µM RU-486 (mean ± SD).

(D and E) Expression of irg1 within infected control MO-injected and Nr3c1-depleted larvae, respectively, at 1 hpi.

(F) Quantification of irg1⁺ cells within individual larvae as shown in (D) and (E) (mean ± SD).

(G and H) Expression of cebpβ within infected control MO-injected and Nr3c1-depleted larvae, respectively, at 1 hpi.

(I) Immunofluorescence detection of Nr3c1 (Alexa 546) and Mpeg1/EGFP (Alexa 488) within DAPI-stained infected macrophage-lineage marking Tg(mpeg1:EGFP) larvae at 1 hpi. Dashed line marks Nr3c1⁺ nucleus.

(J) Expression of irg1 and cebpβ within dexamethasone-, hydrocortisone-, and prednisolone-injected larvae at 2 hpi.

(K) Quantification of irg1⁺ cells within individual larvae as shown in (J) (mean ± SD).

(L) Schematic illustrating proposed regulation of infection-responsive irg1 expression within macrophage-lineage cells. Asterisks, arrows, and arrowheads mark blood-specific, liver-specific, and gut-specific cebpβ expression, respectively. All views anterior to left. Numbers represent frequency of larvae with displayed phenotype. Scale bar, 100 µm in (A). Abbreviations: p < 0.001; p < 0.0001; n.s., not significant. See also Figure S3.

Expression Data

Genes:	acod1 cebpb EGFP
Fish:	WT WT + MO4-nr3c1 gl22Tg
Conditions:	chemical treatment: dexamethasone chemical treatment: cortisol chemical treatment: prednisolone bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium, chemical treatment: mifepristone
Knockdown Reagent:	MO4-nr3c1
Anatomical Terms:	blood macrophage intestine liver macrophage
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO4-nr3c1
Conditions:	chemical treatment: dexamethasone chemical treatment: cortisol chemical treatment: prednisolone bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium, chemical treatment: mifepristone
Knockdown Reagent:	MO4-nr3c1
Observed In:	blood macrophage macrophage
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Cell Metabolism, 18(2), Hall, C.J., Boyle, R.H., Astin, J.W., Flores, M.V., Oehlers, S.H., Sanderson, L.E., Ellett, F., Lieschke, G.J., Crosier, K.E., and Crosier, P.S., Immunoresponsive Gene 1 Augments Bactericidal Activity of Macrophage-Lineage Cells by Regulating β-Oxidation-Dependent Mitochondrial ROS Production, 265-278, Copyright (2013) with permission from Elsevier. Full text @ Cell Metab.