Manipulation of Gata3 mainly changes the development of rostral PPR. A–H: In gata3 morphants, reduced olfactory cxcr4b (B) and lens foxe3 (D) are observed compared with controls (A,C). Slightly reduced otic primordial pax2a (F) and epibranchial primordial sox3 (H) expressions are observed compared with their corresponding controls (E,G). I–L: Knocking down gata3 function with an upcMO at 6 hpf, the lens foxe3 (J) is slightly smaller than that of control (I). The OEPD pax2a is essentially unchanged (K,L). Embryos are injected with double-stranded MO at one-cell stage and cleaved by UV light at 6 hpf. M–P: Mis-expression of gata3 by heat shocking a transgenic line Tg(gfp:hse:gata3) at 6 hpf expands the lens foxe3 (N), but not the OEPD pax2a (P), compared with their controls (M,O). Q,R: Expression of six4.1 is reduced when Gata3 function is knocked down using upcMO. Embryos are injected at one-cell stage and treated by UV light at 6 hpf. S,T: Over expression of gata3 by heat shocking a transgenic line Tg(gfp:hse:gata3) at 6 hpf enhances six4.1 expression. U,V: Knocking down Gata3 function reduces the PPR six4.1 expression. W,X: Over expression of gata3 by mRNA injection enhances the PPR six4.1. A–D, I–J, and M–N: Side views with anterior to the left; E–H, K–L, and O–X: Dorsal views with anterior to the left. A–D, I–J, and M–N: 24 hpf; E–F and K–J: 12 hpf; G–H, Q–X: 11 hpf; O–P 12.5 hpf. Scale bar = 200 µm.
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