FIGURE

Fig. 1

ID

ZDB-FIG-150430-3

Publication

Moritz et al., 2015 - Live imaging reveals a new role for the sigma-1 (σ1) receptor in allowing microglia to leave brain injuries

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Fig. 1

σ₁ Receptor is important for microglial reaction toward injury.

(A) Percentage of microglia reacting to injury in DMSO compared to DTG and PB190 treated zebrafish larvae. (B) Speed of microglia branch extension and retraction in DMSO, DTG and PB190 treated embryos. (C) Time course of microglia (pU1::Gal4UAS::TagRFP) reaction toward injuries in DMSO treated embryos. (C1) before, (C2) 10 min after and (C3) 90 min after cut. (D) Time course of microglia (pU1::Gal4UAS::TagRFP) reaction toward injuries in PB190 treated embryos. (D1) before, (D2) 10 min after and (D3) 90 min after cut. (E) Dorsal views of a 3,5 dpf larval brain showing the time course of a Ca²⁺ wave (betaactin::GCaMP3.1) forming upon central brain injury in PB190 treated zebrafish larvae. (E1) before, (E2) 2 s after and (E3) 12 s after cut. Scalebar for all images 20 µm. Images (C) and (D) were obtained using an Andor Spinning Disk Confocal with a 40x/NA1.15 objective. Images (E) were produced using an Olympus FV 1000 with a 40x/NA1.15 objective. Statistical analysis was performed using a KruskalWallis test with a Dunn’s multiple comparison test.

Expression Data

Genes:	GCaMP RFP
Fish:	hdb1Tg hdb2Tg
Conditions:	physical alteration: brain chemical treatment: agonist, physical alteration: brain
Anatomical Terms:	brain brain glial cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hdb1Tg hdb2Tg
Condition:	chemical treatment: agonist, physical alteration: brain
Observed In:	brain calcium-mediated signaling glial cell response to wounding
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neurosci. Lett.