Fig. 1

Dynasore treatment impairs epiboly of the deep cells and EVL. ((A)(C)) Live images of a representative untreated ((A)(A′′)), DMSO control treated ((B)(B′′)) and dynasore treated embryo ((C)(C′′)) at epiboly initiation ((A)(C)), mid-epiboly ((A2)(C2)) and late epiboly progression ((A′′)(C′′)). Arrowhead in (C) indicates uneven doming of the blastoderm, arrowheads in ((A′)(C2)) indicate the embryonic shield and arrows in ((A′′)(C′′)) mark the approximate position of the advancing blastoderm margin. ((D)(F)) Phalloidin (red) and sytox (green) co-staining showing the effect of dynasore treatment (F) on epiboly of the deep cell margin (DCM), EVL and YSL (marked by the YSN). Arrowheads in (F) show the advanced position of YSN ahead of the blastoderm margin. (G) Quantification of the effect of dynasore treatment on epiboly progression. Bars represent the mean percentage of yolk coverage (n=10). Error bars indicate standard deviation and statistical significance was determined by t-test, ^*P<0.01. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)