FIGURE

Fig. 5

ID

ZDB-FIG-150415-7

Publication

Ingold et al., 2015 - Proper migration and axon outgrowth of zebrafish cranial motoneuron subpopulations require the cell adhesion molecule MDGA2A

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Fig. 5

Representative images from control (w) and MDGA2A morpholino treated (mA and mB) embryos are shown. 28 to 34h post fertilization facial neurons in wt embryos project axons along a predetermined path, displaying a well-documented 60° turn (asterisks). In MDGA2A morphants this turning angle is absent or strongly reduced and defasciculation events occur much more frequently. Analyzing fluorescence intensity below the main axon bundle of the facial nerve indicates that fluorescence in MDGA2A knockdown embryos is slightly increased, supporting the finding of increased defasciculation and axon branching of facial neurons (see supplementary material Fig. S3D–F). The scale bar represents 20µm.

Expression Data

Gene:	GFP
Fish:	rw0Tg rw0Tg + MO1-mdga2a
Knockdown Reagent:	MO1-mdga2a
Anatomical Terms:	cranial nerve VII facial nerve motor nucleus branchiomotor neuron
Stage Range:	Prim-5 to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rw0Tg + MO1-mdga2a
Knockdown Reagent:	MO1-mdga2a
Observed In:	cranial nerve VII axon cranial nerve VII collateral sprouting in absence of injury facial nerve formation
Stage Range:	Prim-5 to Prim-15

Phenotype Detail

Acknowledgments

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