FIGURE

Fig. 2

ID

ZDB-FIG-150408-47

Publication

Keßler et al., 2015 - Loss of dihydrolipoyl succinyltransferase (DLST) leads to reduced resting heart rate in the zebrafish

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Fig. 2

ste encodes for dihydrolipoyl succinyltransferase (dlst). a Integrated genetic and physical map of the ste locus. The ste mutation interval is flanked by the microsatellite markers z13385 and z381 and encodes two open reading frames, zebrafish dlst and prox2. b Within the coding sequence of zdlst a point mutation (G→A) was identified at the splice donor site of intron 5 leading to aberrant pre-mRNA splicing and the premature termination of DLST translation. An arrow marks the mutated base. c, d Spatial expression of dlst by whole-mount antisense RNA in situ hybridization of wt and ste^−/− embryos at 72 hpf. cdlst is ubiquitously expressed with pronounced levels in the brain, skeletal muscle, fins and heart of wild-type embryos. d Strongly reduced dlst mRNA levels in ste^−/− mutants compared to wild-type littermates. e Quantitative real-time PCR of ste^−/− mutants and wild-type siblings. Nonsense mediated dlst-RNA decay in ste^−/− mutants compared to wild-type siblings at 72 hpf

Expression Data

Gene:	dlst
Fish:	WT dlst^{t21498/t21498}
Anatomical Terms:	brain fin heart skeletal muscle whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Basic Res. Cardiol.