FIGURE

Fig. 3

ID

ZDB-FIG-150402-20

Publication

Wan et al., 2014 - Retinal Injury, Growth Factors, and Cytokines Converge on β-Catenin and pStat3 Signaling to Stimulate Retina Regeneration

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Fig. 3

Mapk/Erk, PI3K/Akt, β-Catenin, and Jak/Stat Signaling Regulate Injury and Growth Factor-Stimulated MG Proliferation

(A and B) Mapk/Erk inhibitor (UO126) or PI3K inhibitors (Ly294002 or PI-103) reduce the number of BrdU⁺ progenitors at 4 dpi. Asterisks identify the injury site. Scale bar represents 50 µm. Error bars represent SD; p < 0.001; n = 6.

(D) pErk and pAkt immunofluorescence in uninjured and injured (4 dpi) retinas. Asterisks identify the injury site. In the pErk panels, arrows point to pErk⁺ MG and arrowheads point to BrdU⁺ cells. In pAkt panels, arrowheads point to pAkt⁺ cells, while arrows point to BrdU⁺ cells. Scale bar represents 50 µm.

(E) In the growth factor stimulated, but uninjured retina, BrdU⁺ progenitor formation is suppressed by inhibiting Mapk (UO126), PI3K (Ly294002), Jak/Stat (JSI-124) or β-catenin (pyrvinium) signaling. Error bars represent SD; p < 0.001; n = 5.

See also Figures S2–S4.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Conditions:	chemical treatment: LY294002, perforation: retina chemical treatment: PI-103, perforation: retina chemical treatment: U0126.EtOH, perforation: retina
Observed In:	Muller cell cell population proliferation regenerating tissue Muller cell retina tissue regeneration
Stage:	Adult

Phenotype Detail

Acknowledgments

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