PUBLICATION

Retinal Injury, Growth Factors, and Cytokines Converge on β-Catenin and pStat3 Signaling to Stimulate Retina Regeneration

Authors
Wan, J., Zhao, X.F., Vojtek, A., Goldman, D.
ID
ZDB-PUB-140930-8
Date
2014
Source
Cell Reports   9(1): 285-97 (Journal)
Registered Authors
Goldman, Dan, Wang,Jin
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cell Differentiation/physiology
  • Cytokines/metabolism*
  • Intercellular Signaling Peptides and Proteins/metabolism*
  • Nerve Regeneration/physiology*
  • Retina/injuries
  • Retina/metabolism*
  • Retina/physiology*
  • STAT3 Transcription Factor/metabolism*
  • Signal Transduction
  • Zebrafish
  • Zebrafish Proteins/metabolism*
  • beta Catenin/metabolism*
PubMed
25263555 Full text @ Cell Rep.
Abstract
Müller glia (MG) in the zebrafish retina respond to retinal injury by generating multipotent progenitors for retinal repair. Here, we show that Insulin, Igf-1, and fibroblast growth factor (FGF) signaling components are necessary for retina regeneration. Interestingly, these factors synergize with each other and with heparin-binding EGF-like growth factor (HB-EGF) and cytokines to stimulate MG to generate multipotent progenitors in the uninjured retina. These factors act by stimulating a core set of signaling cascades (Mapk/Erk, phosphatidylinositol 3-kinase [PI3K], β-catenin, and pStat3) that are also shared with retinal injury and exhibit a remarkable amount of crosstalk. Our studies suggest that MG both produce and respond to factors that stimulate MG reprogramming and proliferation following retinal injury. The identification of a core set of regeneration-associated signaling pathways required for MG reprogramming not only furthers our understanding of retina regeneration in fish but also suggests targets for enhancing regeneration in mammals.
Genes / Markers
Figures
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping