FIGURE

Fig. 3

ID
ZDB-FIG-150330-32
Publication
Fukui et al., 2014 - S1P-Yap1 Signaling Regulates Endoderm Formation Required for Cardiac Precursor Cell Migration in Zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 3

Yap1 Regulates Endodermal Cell Survival

(A) Time-sequential (from eight-somite stage [8s] to 16-somite stage [16s]) images of Tg(sox17:GFP) embryos injected with MO indicated at the left. Arrows denote the fragmentation of sox17 promoter-driven GFP-positive cells. A set of representative images of eight independent experiments is shown. Dorsal view.

(B) Incidence of cardia bifia (black) and normal heart (white) in the morphants exhibiting less than 5 fragmented endodermal cells or more than 5 fragmented endodermal cells in the embryos treated with the MO indicated at the bottom.

(C) Bar graph shows the number of the TUNEL-positive cells among the GFP-positive endodermal cells of Tg(sox17:GFP) embryos at 14-somite stage (14s) injected with MO indicated at the bottom. ctrl MO, control morpholino. p < 0.01 (versus ctrl morphants).

(D) Bar graph shows the number of the TUNEL-positive cells in a S1pr2 mutant (mil), heterozygous embryo (s1pr2m93/+) and homozygous embryos (s1pr2m93/m93). p < 0.01.

(E) Gene expression profile analyzed by RNA-seq using RNAs obtained from the Tg(sox17:GFP) embryos uninjected (control) and injected with yap1 MO by sorting of GFP-positive endodermal cells. The graph shows the ratio of the reads per kilobase per million reads (RPKM) of the yap1 morphants-derived RNAs divided by that of the control. Among the genes we analyzed, those regulating cell survival are listed.

See also Figure S3 and Movies S1, S2, and S3.

Expression Data
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: 5-9 somites to 14-19 somites

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: 14-19 somites

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 31, Fukui, H., Terai, K., Nakajima, H., Chiba, A., Fukuhara, S., Mochizuki, N., S1P-Yap1 Signaling Regulates Endoderm Formation Required for Cardiac Precursor Cell Migration in Zebrafish, 128-136, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell