FIGURE

Fig. 3

ID

ZDB-FIG-150326-33

Publication

Dyer et al., 2014 - Specification of sensory neurons occurs through diverse developmental programs functioning in the brain and spinal cord

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Fig. 3

Notch signalling components are expressed in the midbrain at early neurogenesis stages. Lateral views of the heads of 14-hpf embryos processed by in situ hybridisation (A–V). The Notch receptors elta, deltaB, deltaC, and deltaD (A–H), the Notch ligands notch1a, notch1b, notch2, and notch3 (I–P) and Notch-regulated genes her4, hey21, and hey4 (Q-V) were assessed for expression in the dorsal midbrain where MTN neurons will arise (bracket). No specific delta gene expression was detected, but notch1b and notch2 expression was observed in the dorsal midbrain. Both hey2 and her4 also showed expression in dorsal midbrain cells. Dorsal and orthogonal views of a 24-hpf Tg[elavl3:egfp] embryo processed by fluorescent in situ hybridisation and immunohistochemistry with anti-GFP reveals that expression of deltaB did not co-localise with EGFP in MTN neurons (intersect lines, W). Scale bars = 100 µm (A–W).

Expression Data

Genes:	dla dlb dlc dld EGFP her4.1 hey1 hey2 notch1a notch1b notch2 notch3
Fish:	WT knu3Tg
Anatomical Terms:	brain diencephalon ventral region hindbrain mesencephalic nucleus of trigeminal nerve midbrain midbrain posterior region midbrain postero-ventral region telencephalon antero-dorsal region telencephalon dorsal margin telencephalon ventral margin telencephalon ventral region thalamus
Stage Range:	10-13 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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