FIGURE

Fig. 2

ID

ZDB-FIG-150326-32

Publication

Dyer et al., 2014 - Specification of sensory neurons occurs through diverse developmental programs functioning in the brain and spinal cord

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Fig. 2

MTN neurons do not arise from neural crest and do not require sox10 function for their development. Expression (blue) of isl1 (A,C) and drg11 (B) in MTN (arrows) and nTPC neurons does not overlap with expression of foxd3 (A,B) and sox10 (C) in mesencephalic neural crest (red) in 24-hpf embryos processed by double in situ hybridisation. Similarly, there is no co-localisation of Isl1 protein (D,F) or drg11 expression (G,I) in MTN neurons with EGFP (E,F,H,I) in neural crest of 24-hpf Tg[foxd3:egfp] embryos, although there is co-localisation in the epiphysis (e). There is no apparent difference in the number of isl1 expressing MTN neurons (J,K) or RB neurons (L,M) in cls mutants (K,M) relative to wildtype (WT) siblings (J,L) at 26 hpf. Quantification of RB, MTN, and nTPC neurons in cls and WT embryos reveals no significant (NS) differences except for a difference in nTPC number (*P<0.05, error bars represent standard error of the mean, N). Scale bars = 100 µm (A–M).

Expression Data

Genes:	drgx foxd3 GFP isl1a sox10
Antibody:	Ab2-isl
Fish:	WT sox10^baz1/baz1 zf15Tg
Anatomical Terms:	epiphysis mesencephalic nucleus of trigeminal nerve neural crest neural crest midbrain nucleus of the caudal commissure Rohon-Beard neuron
Stage Range:	20-25 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sox10^baz1/baz1
Observed In:	nucleus of the caudal commissure
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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