FIGURE

Fig. 8

ID

ZDB-FIG-150325-19

Publication

Mommaerts et al., 2014 - Smoc2 modulates embryonic myelopoiesis during zebrafish development

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Fig. 8

smoc2 morphants display defects in embryonic myelopoiesis at the onset of blood circulation. A–D: Lateral views of the embryos, anterior to the left, dorsal to the top, at the onset of circulation. A: WISH analysis or the macrophage markers csf1ra (A) and irf8 (B), and the neutrophil markers c/ebp1 (C) and mpx (D). E–G: qPCR analysis for irf8 (E), c/ebp1 (F) and mpx (G). Values plotted as mean ± SEM; n = 4; *P < 0.05. H,I: Visualization of the mpx-positive cells using the mpx reporter embryos (Tg(mpx:GFP)ⁱ¹¹⁴) at 3 dpf (H) and the quantification of the number of neutrophils in the ATG morphants (n = 42) as compared to the control embryos (n = 12; *P < 0.05) (I).

Expression Data

Genes:	cebp1 csf1ra GFP irf8 mpx
Fish:	WT WT + MO4-tp53 + MO5-smoc2 i114Tg i114Tg + MO4-tp53 + MO5-smoc2
Knockdown Reagents:	MO4-tp53 MO5-smoc2
Anatomical Terms:	macrophage neutrophil
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO4-tp53 + MO5-smoc2 i114Tg + MO4-tp53 + MO5-smoc2
Knockdown Reagents:	MO4-tp53 MO5-smoc2
Observed In:	myeloid cell development neutrophil
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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