FIGURE

Fig. 3

ID

ZDB-FIG-150320-15

Publication

Coxam et al., 2015 - carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad) regulates Notch signaling and vascular development in zebrafish

Other Figures

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Fig. 3

cad artery hyperbranching is Vegfc/Vegfr3 dependent. A,B: The vasculature Tg(fli1a:EGFP) in wild-type sibling (A) and cad^hu10125 (B) embryos at 3 dpf. C–E: The vasculature Tg(fli1a:EGFP) in cad^hu10125/MO-vegfc (C), cad^hu10125/MO-ccbe1 (D), and cad^hu10125/MO-vegfr3 (E) embryos at 3 dpf. F: Quantification of the severity of vascular hyperbranching designated as mild or severe (where mild: 1–7 hyperbranched arteries, and severe: e 8 hyperbranched arteries along the entire embryo body axis) scored in WT (n = 16), MO-vegfc (n = 12), MO-ccbe1 (n = 10) and MO-vegfr3 (n = 14) injected cad^hu10125 embryos. G,H: The thymidylate synthase mutant tyms^hi3510Tg does not present with arterial hyperbranching. G: Overall morphology of wild-type (n = 30/30) and tyms^hi3510Tg (n = 30/30) embryos at 4 dpf. H: The vasculature Tg(fli1a:EGFP) in wild-type (n = 30/30) and tyms^hi3510Tg (n = 30/30) embryos at 4dpf. White arrowhead: thoracic duct, white arrow: example of normal aISV.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tyms^{hi3510Tg/hi3510Tg} tyms^{hi3510Tg/hi3510Tg}; y1Tg cad^{hu10125/hu10125} + MO1-ccbe1 cad^{hu10125/hu10125} + MO1-vegfc cad^{hu10125/hu10125} + MO3-flt4
Knockdown Reagents:	MO1-ccbe1 MO1-vegfc MO3-flt4
Observed In:	eye head intersegmental artery
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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