Fig. 4

Purkinje-specific GFF and membrane tagged Venus/mCherry lines. (A–H) aldoca:gap43-Venus larvae (A–D, dorsal views with anterior to the left) and adult sagittal sections (E–H, with anterior to the left) were co-stained with anti-GFP (Venus, green) and anti-Pvalb7 (magenta) antibodies. Venus signals colocalized with Pvalb7 signals in all the Purkinje cells of 5-dpf larvae (D) and the adult cerebellum (H). Cerebellovestibular tracts are indicated by arrowheads (A, B). (I–L) aldoca:gap43-mCherry larvae (dorsal views with anterior to the left) were co-stained with anti-DsRed (mCherry, magenta) and anti-Pvalb7 (green) antibodies. (M–S) aldoca:GFF; UAS:GFP larvae (M–P, dorsal views with anterior to the left) and adult sagittal sections (Q–V, anterior to the left) were co-stained with anti-GFP (Venus, green) and anti-Pvalb7 (magenta) antibodies. (T–V) High magnification views of the box in S. Only a portion of the Pvalb7⁺ Purkinje cells stained positive for GFP (L, P). Scale bars: 100 µm in A; 50 µm in D (applied to B–D); 200 µm in E; 50 µm in H (applied to F–H); 100 µm in I; 50 µm in L (applied to J–L); 100 µm in M; 50 µm in P (applied to N–P); 200 µm in S (applied to Q–S); 100 µm in V (applied to T–V).