FIGURE

Fig. 2

ID

ZDB-FIG-150319-6

Publication

Takeuchi et al., 2015 - Establishment of Gal4 transgenic zebrafish lines for analysis of development of cerebellar neural circuitry

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Fig. 2

Granule-specific GFF and GFP transgenic lines. (A, B) gSA2AzGFF152B; UAS:GFP. (C, D) gSAIzGFFM765B; UAS:GFP. (E, F) gSAG6A. (G, H) gSAIGFF23C; UAS:GFP. (I, J) SAGFF(LF)128A; UAS:GFP. (K, L) hspGFF57A; UAS:GFP. (M, N) SAGFF(LF)157B; UAS:GFP. (O, P) hspGFFDMC90A; UAS:GFP. To visualize GFF expression, the granule-specific GFF lines (except gSAG6A in E, F) were crossed with the UAS:GFP line. The 5-dpf larvae (A, C, E, G, I, K, M, O, dorsal projection views with anterior to the left) and sagittal sections of adult brains (B, D, F, H, J, L, N, P, optical sections with anterior to the left) were stained with anti-GFP antibody. Note that in the gSA2AzGFF152B; UAS:GFP line GFP was specifically expressed in the granule cells of the CCe, while in the gSAIzGFFM765B; UAS:GFP line GFP was expressed in some granule cells in the CCe and most granule cells in the LCa in adult. The gSAG6A and gSAIGFF23C; UAS:GFP lines expressed GFP in granule cells primarily in the LCa. In the hspGFFDMC90A; UAS:GFP line GFP was expressed in some granule cells in the GCL and LCa, and in some immature granule cells in the ML. Scale bars: 100 µm in O (applied to A, C, E, G, I, K, M, O); 200 µm in B, D, F, H, J, L, N, P.

Expression Data

Gene:	EGFP
Fish:	nkgSAG6AGt nkgSA2AzGFF152BGt; nkuasgfp1aTg nkgSAIGFF23CGt; nkuasgfp1aTg nkgsaizGFFM765BGt/nkgsaizGFFM765BGt; nkuasgfp1aTg nkhspGFF57AEt; nkuasgfp1aTg nkhspGFFDMC90AEt; nkuasgfp1aTg nkSAGFFLF128AEt; nkuasgfp1aTg nkSAGFFLF157BEt; nkuasgfp1aTg
Condition:	heat shock
Anatomical Terms:	cerebellar crest cerebellar granule cell cerebellar granule cell corpus cerebelli cerebellar granule cell granular layer corpus cerebelli cerebellar granule cell granular layer valvula cerebelli cerebellar granule cell medial caudal lobe cerebellar granule cell valvula cerebelli cerebellar granule cell
Stage Range:	Day 5 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 397(1), Takeuchi, M., Matsuda, K., Yamaguchi, S., Asakawa, K., Miyasaka, N., Lal, P., Yoshihara, Y., Koga, A., Kawakami, K., Shimizu, T., Hibi, M., Establishment of Gal4 transgenic zebrafish lines for analysis of development of cerebellar neural circuitry, 1-17, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.