Fig. 8

Cilia structure and function are normal in kif6^gw326 mutants. A: Defects in left–right asymmetry were evaluated by visualizing the position of the heart in 30 hpf embryos for WT (N = 94) and kif6^gw326 mutants (N = 173). Normally, zebrafish hearts are located to the left of the midline (right side when viewed ventrally) at 30 hpf. In zebrafish with ambiguous heart positioning, hearts appear at the midline and to the right side (left side when viewed ventrally) in zebrafish with situs inversus. Ambiguous and inversed orientations of the heart were observed at similar frequencies in WT and kif6^gw326 embryos. B: Otoliths were observed laterally in 48–72 hpf zebrafish embryos. Normal otolith numbers (two) and abnormal otolith numbers (one or three) were quantified WT (N = 371) and kif6^gw326 mutants (N = 305). C: In situ hybridization for southpaw (spw) to highlight LR patterning and no tail (ntl) to highlight the midline shows no defect in left–right patterning in kif6 sko/sko mutants (n = 39 WT and 90 kif6 sko/sko mutants). D: In situ hybridization for insulin (ins) to highlight LR patterning in the pancreas shows no defect in the normally rightward place of the pancreas in kif6 sko/sko mutants (n = 11 WT and 34 kif6 sko/sko mutants). E: Whole-mount confocal immunostaining of 28 hpf WT and kif6^gw326 mutant embryos shows normal appearance of cilial in the central canal (arrows). F: Live 48 hpf WT and kif6^gw326 embryos were injected with 5% tetramethylrhodamine conjugated to 70,000 molecular weight dextran. Sixty minutes after injection, the dye has migrated similar distances in skolios mutants compared with WT embryos.