FIGURE

Fig. S5

ID

ZDB-FIG-150304-37

Publication

Campbell et al., 2013 - Local caspase activation interacts with Slit-Robo signaling to restrict axonal arborization

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Fig. S5

casp-3aMO1 and casp-9MO1 morphants do not have increased apoptosis, and RGC axons follow normal trajectories to the optic tectum. (A–F) Lateral views of TUNEL labeling of WT (A), coMO (B), HA14-1 (activates Caspase-3 by the inhibition of Bcl-2, positive control; C), control (vehicle, Milli-Q water; D), casp-3aMO1 (E), and casp-9MO1 (F) at 80 hpf. No increase in apoptosis is observed in the morphants. (G–J) Lateral views of WT (G), coMO (H), casp-3aMO1 (I), and casp-9MO1 (J) morphants at 80 hpf, illustrating the pathway taken by one or a few YFP-Rab3–labeled RGCs from the optic chiasm to the tectum. Those morphant RGC axons that reach the tectum follow a normal trajectory. n = 5 embryos analyzed per condition. Tec, tectum. Anterior is left, and dorsal is up. Bar, 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-casp3a WT + MO2-casp9
Knockdown Reagents:	MO1-casp3a MO2-casp9
Observed In:	brain apoptotic process eye apoptotic process retinal ganglion cell retinal ganglion cell axon guidance
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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