Fig. 6

Anxa4 is cell-autonomously required for liver cell viability. (a) Diagram depicts endoderm transplantation strategy. Donor embryo is injected with sox32mRNA to drive endoderm fate, rhodamine dextran for labeling, and MOs to knock down gene expression. At 4 hpf, donor cells are transplanted into a host embryo, where they will contribute primarily to the host endoderm. (b) The mosaic 35 hpf host embryo obtained after the endoderm transplantation contains rhodamine dextran labeled donor endoderm cells (white arrows). (c) and (d′) The ventral view of 48 hpf Tg(sox17:GFP) foregut with rhodamine dextran labeled WT (white arrowheads in (c) and (c′) or anxa4 morphant donor cells (d) and (d′). L, liver; P, pancreas. Note that rhodamine labeling (white arrowheads in (d) and (d′)) appears scattered and not restricted within individual cells in the host embryo with Anxa4 morphant donor cells. (e)-(g′′) A single optical section (ventral view) of a 31 hpf mosaic host embryo with Anxa4 morphant cells from a Tg(sox17:GFP) donor embryo showing GFP blebs in the liver (upper inset and (f) and (f′′) but not dorsal pancreas (lower inset and (g) and (g′′). Subsets of GFP positive morphant donor cells are also positive for the cleaved Caspase 3 (white arrows) in the liver but not the dorsal pancreas. L, liver; dP, dorsal pancreas.