Fig. 4

Conserved zebrafish and mouse Anxa4 expression in the liver and pancreas. (a)-(g′) Immunofluorescent labeling of Anxa4 in the liver and pancreas of E12, E14, and P25 mice. From E12 to E14, Anxa4 is detected in Prox1⁺ liver cells (a) and (c), and in all pancreatic cells (b) and (b′′); (d) and (d′′), including Sox9⁺ or Glucagon⁺ cells and cells negative for both Sox9 and Glucagon (white arrowheads in b-b′′). At E14, both the Sox9 high (yellow arrowheads) and low (blue arrowheads) expression cells are positive for Anxa4. In adult mice, Anxa4 is expressed the intrahepatic biliary ducts (e) and (e′); white arrowheads) and intrapancreatic ducts (f) and (f′); white arrowheads), co-localizing with ductal expression of E-Cadherin. Anxa4 is also expressed in pancreatic endocrine islets (g) and (g′), with localization to the membrane (white arrowhead) and nuclear envelope (yellow arrowhead). Low level Anxa4 is expressed in acinar cells (blue arrowheads) (h)-(j′) Immunofluorescent labeling of Anxa4 in the zebrafish liver (h) and pancreas (I) of one-year old Tg(ptf1a:GFP);Tg(lfabf:ds-Red), and pancreas of 2-month old Tg(ins:dsRed);Tg(gcga:GFP), showing Anxa4 is expressed in the networks of intrahepatic (h) and intrapancreatic (i) and (j′) ducts as well as pancreatic islets (j), (j′) single optical section, white arrowheads).