FIGURE

Fig. 3

ID

ZDB-FIG-150129-11

Publication

Zhao et al., 2014 - Leptin and IL-6 Family Cytokines Synergize to Stimulate Müller Glia Reprogramming and Retina Regeneration

Other Figures

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Fig. 3

Jak/Stat3 Signaling Mediates Injury-Dependent Induction of the Reprogramming Gene ascl1a

(A) GFP immunofluorescence in 6-ascl1a:gfp fish shows that the Jak inhibitors P6 and JSI-124, applied at the time of retinal injury, inhibit injury-dependent transgene induction.

(B) Diagram of the ascl1a promoter constructs used to generate transgenic lines.

(C) GFP immunofluorescence shows that a distal 1.5 kb fragment of the ascl1a promoter is required for injury-dependent transgene expression and that both consensus Stat3 sites located in this promoter fragment are necessary for this expression. BrdU⁺ cells indicate the injury site and a normal regenerative response. The asterisk marks the injury site (needle poke). Scale bar, 50 µm.

See also Figure S4.

Expression Data

Gene:	EGFP
Fish:	mi31Tg mi32Tg mi33Tg mi37Tg
Conditions:	physical alteration: retina chemical treatment: pharmaceutical, physical alteration: retina chemical treatment: pharmaceutical, physical alteration: retina
Anatomical Term:	Muller cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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