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Fig. 3
Jak/Stat3 Signaling Mediates Injury-Dependent Induction of the Reprogramming Gene ascl1a
(A) GFP immunofluorescence in 6-ascl1a:gfp fish shows that the Jak inhibitors P6 and JSI-124, applied at the time of retinal injury, inhibit injury-dependent transgene induction.
(B) Diagram of the ascl1a promoter constructs used to generate transgenic lines.
(C) GFP immunofluorescence shows that a distal 1.5 kb fragment of the ascl1a promoter is required for injury-dependent transgene expression and that both consensus Stat3 sites located in this promoter fragment are necessary for this expression. BrdU+ cells indicate the injury site and a normal regenerative response. The asterisk marks the injury site (needle poke). Scale bar, 50 µm.
See also Figure S4.