FIGURE

Fig. 2

ID

ZDB-FIG-150115-21

Publication

Imai et al., 2014 - Stem-loop binding protein is required for retinal cell proliferation, neurogenesis, and intraretinal axon pathfinding in zebrafish

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Fig. 2

Retinal cell differentiation is delayed in the rw440 mutant. (A and B) Wild-type (A) and rw440 mutant (B) retinas labeled with rx1 RNA probe (blue) at 1 dpf. (C and D) Wild-type (C) and rw440 mutant (D) retinas labeled with ath5:GFP (green) at 2 dpf. In wild-type retina, ath5:GFP is strongly expressed in the RGCs and weakly expressed in other retinal neurons such as amacrine cells and ONL cells. In the rw440 mutant retina, a weak expression of ath5:GFP is observed in the central retina. (E and F) Wild-type (E) and the rw440 mutant (F) retinas labeled with zn5 antibody (green) at 2 dpf. The zn5 antibody labels RGCs in the wild type. In the rw440 mutant, a small number of cells express zn5 (arrowheads, F). (G and H) Wild-type (G) and rw440 mutant (H) retinas labeled with zn5 antibody (green) and phalloidin (magenta) at 3 dpf. Phalloidin labels the IPL and the OPL, making the ONL evident. In the rw440 mutant retina, zn5-labeled RGCs increase (arrowheads), but there is no OPL. (I and J) Wild-type (I) and rw440 mutant (J) retinas labeled with zpr1 antibody (magenta) at 3 dpf. Nuclei were labeled with Sytox Green (green). The zpr1 antibody labels double cone photoreceptors (PR). In the rw440 mutant retina, there is no zpr1 signal. (K and L) Wild-type (K) and the rw440 mutant (L) retinas labeled with anti-Pax6 antibody (green) at 4 dpf. RGCs and amacrine cells express Pax6. In the rw440 mutant retina, presumptive RGCs and some INL cells express Pax6 (arrows, L). (M and N) Wild-type (M) and the rw440 (N) mutant retinas labeled with zpr1 antibody (magenta) at 4 dpf. In the rw440 mutant retina, some ONL cells express zpr1 (arrowheads, N) in clusters. (O and P) Wild-type (O) and the rw440 mutant (P) retinas labeled with anti-Pax6 antibody (green) at 5 dpf. In the rw440 mutant retina, presumptive RGCs and some ONL cells express Pax6 (arrows, P). (Q and R) Wild-type (Q) and the rw440 mutant (R) retinas labeled with zpr1 antibody (magenta) at 5 dpf. In the rw440 mutant retina, clustered ONL cells express zpr1 (arrowheads, R). RGC, retinal ganglion cell; ONL, outer plexiform layer; PR, photoreceptor.

Expression Data

Gene:	GFP
Antibodies:	Ab1-pax6 zn-5 zpr-1
Fish:	rw021Tg slbp^rw440/rw440; rw021Tg
Anatomical Terms:	amacrine cell retina central region retinal bipolar neuron retinal cone cell retinal ganglion cell retinal inner nuclear layer retinal outer nuclear layer
Stage Range:	Long-pec to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slbp^rw440/rw440; rw021Tg
Observed In:	eye photoreceptor cell differentiation retinal ganglion cell retinal outer nuclear layer
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 394(1), Imai, F., Yoshizawa, A., Matsuzaki, A., Oguri, E., Araragi, M., Nishiwaki, Y., Masai, I., Stem-loop binding protein is required for retinal cell proliferation, neurogenesis, and intraretinal axon pathfinding in zebrafish, 94-109, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.