FIGURE

Fig. 4

ID

ZDB-FIG-141231-3

Publication

Schulte et al., 2014 - Targeted resequencing and systematic in vivo functional testing identifies rare variants in MEIS1 as significant contributors to restless legs syndrome

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Fig. 4

Functional Assessment of Null and Benign MEIS1 Variants by In Vivo Complementation in Zebrafish Embryos and Evaluation of Hindbrain Patterning

(A-E) At 14-16 hpf, developing zebrafish embryos were evaluated for the integrity of rhombomeres 3 and 5 (r3 and r5) by in situ hybridization with a riboprobe against krox20. Upon disruption of meis1, we observed rhombomeric defects that involved widening of the evaluated structures (B and D) or shortening of the distance between r3 and r5 (D), as well as thinning or absence of the evaluated structures.

(F) Quantification showing that the aberrant phenotypes were especially pronounced in the morphant embryos and embryos coinjected with MO+null mRNA (n ≥ 26 embryos per genotype). Abbreviations are as follows: MO, morpholino; WT, wild-type.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-meis1
Knockdown Reagent:	MO1-meis1
Observed In:	hindbrain morphogenesis rhombomere 3 rhombomere 5
Stage:	10-13 somites

Phenotype Detail

Acknowledgments

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