FIGURE

Fig. 5

ID

ZDB-FIG-141218-4

Publication

Lai et al., 2014 - Zebrafish WNK Lysine Deficient Protein Kinase 1 (wnk1) Affects Angiogenesis Associated with VEGF Signaling

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Fig. 5

Effect of wnk1a or wnk1b knockdown on vasculogenesis and angiogenesis in Tg(fli1:GFP) embryos.

(A, B) Whole mount in situ hybridization for etv2 at 14 and 18 hpf in uninjected control embryos (A1, B1), wnk1a morphants (A2, B2), and wnk1b morphants (A3, B3). Flat mounts of de-yolked embryos were prepared. (C) GFP fluorescence was used to assay ISV formation in uninjected control embryos (C1), wnk1a morphants (C2), and wnk1b morphants (C3) at 33 hpf. (D1) etv2 expression in uninjected control, wnk1a and wnk1b morphants as a percentage of embryos exhibit strong or expression, (D2) ISV length in wnk1a and wnk1b morphants.

Expression Data

Genes:	EGFP etsrp
Fish:	WT WT + MO1-wnk1a WT + MO4-wnk1b y1Tg y1Tg + MO1-wnk1a y1Tg + MO4-wnk1b
Knockdown Reagents:	MO1-wnk1a MO4-wnk1b
Anatomical Terms:	blood vessel endothelial cell cranial vasculature intersegmental vessel trunk vasculature
Stage Range:	10-13 somites to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg + MO1-wnk1a y1Tg + MO4-wnk1b
Knockdown Reagents:	MO1-wnk1a MO4-wnk1b
Observed In:	intersegmental vein intersegmental vessel angiogenesis
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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