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Fig. 5
Effect of wnk1a or wnk1b knockdown on vasculogenesis and angiogenesis in Tg(fli1:GFP) embryos.
(A, B) Whole mount in situ hybridization for etv2 at 14 and 18 hpf in uninjected control embryos (A1, B1), wnk1a morphants (A2, B2), and wnk1b morphants (A3, B3). Flat mounts of de-yolked embryos were prepared. (C) GFP fluorescence was used to assay ISV formation in uninjected control embryos (C1), wnk1a morphants (C2), and wnk1b morphants (C3) at 33 hpf. (D1) etv2 expression in uninjected control, wnk1a and wnk1b morphants as a percentage of embryos exhibit strong or expression, (D2) ISV length in wnk1a and wnk1b morphants.