Fig. S1

Any reduction in kit signaling affects melanocyte regeneration. (A) Schema of the experiments. Fish were reared until 3 dpf and scored for ontogenetic melanocytes (open arrowhead) or treated with 4-HA from 1-3 dpf and scored for regeneration melanocytes at 6 dpf (filled arrowhead). (B) Comparison of kita mutant genotypes with WT. Three to ten embryos were compared with WT controls for ontogenetic melanocytes (empty bars) and regeneration melanocytes (filled bars). Alleles kita^b5 and kita^j1e99 are referred to as kita^null and kit^ats in the main text and figures. Allele kita^j1e60 affects ontogenetic melanocyte migration without affecting ontogenetic melanocyte survival (Rawls and Johnson, 2003). Allele kita^j1e78 affects ontogenetic melanocyte survival without affecting ontogenetic melanocyte migration (Rawls and Johnson, 2003). Experiments with kita^j1e99 were performed at 25°C, and all others were performed at 28.5°C. Error bars indicate s.d. *P<0.05, **P<0.005, ***P<0.001.